Aunt May

May Parker is a character from Marvel Comics. May is Peter Parker's aunt and the widow of Ben Parker. She became the sole legal guardian of Peter after the deaths of his parents and her husband, who was shot by a crook that Peter did not stop. She is often seen as a mother-figure to Peter and cares deeply for him, making it difficult for Peter to hide the fact that he's Spider-Man.

Ultimate Spider-Man
Like her Ultimate counterpart, this version of Aunt May is considerably younger and less frail than her comic book portrayal. She is shown to be involved in various hobbies such as yoga and cooking classes, which allow Peter to sneak out of the house without arousing suspicion. In "Attack of the Beetle", she was asked out to dinner by S.H.I.E.L.D. Agent Phil Coulson (who Nick Fury assigned to be the acting principal of Peter's school). Near the end of Season 3, she reveals she had known for sometime that Peter was Spider-Man, and says she's okay with it and that it changed nothing between them. She is voiced by Misty Lee.

Marvel Cinematic Universe
Aunt May makes her debut appearance in the Marvel Cinematic Universe movie, Captain America: Civil War, portrayed by Marisa Tomei. She will also appear in Spider-Man: Homecoming.

Trivia

 * The Marvel Cinematic Universe version of May is the third portrayal of the character in Marvel's cinematic history. May was previously portrayed by Rosemary Harris and Sally Field in Sony's two Spider-Man film series.