The show tells the heartfelt coming-of-age story of Anne Boonchuy, a self-centered 13-year-old, who is magically transported to the fictitious world of Amphibia, a rural marshland full of frog-people and dangerous creatures. With the help of an excitable young frog named Sprig Plantar, Anne will transform into a hero and discover the first true friendship of her life.
Season two finds Anne and the Plantars on a quest to unlock the mysteries of the music box as they leave the cozy confines of Wartwood for the distant city of Newtopia. On their journey, they will visit exotic new locations, uncover hidden secrets, and experience unexpected reunions as they continue to search for a way to get Anne home.
The third season sees Anne and the Plantars transported from Amphibia to her hometown of Los Angeles, where the frog family will have to learn about the modern world and try to find a way home - all while hiding their identities as talking frog people with Anne’s parents help.
- Brenda Song as Anne Boonchuy
- Justin Felbinger as Sprig Plantar
- Bill Farmer as Hopediah (Hop Pop) Plantar
- Amanda Leighton as Polly Plantar
- Anna Akana as Sasha Waybright
- Haley Tju as Marcy Wu
- Stephen Root as Mayor Toadstool
- Jack McBrayer as Toadie
- Kristen Johnston as Braddock (season 1)
- April Winchell as Fens and Braddock (season 2)
- Matt Jones as Percy
- Darin De Paul as Bog
- Matt Braly as Chuck and Frobo
- Fred Tatasciore as Soggy Joe
- Kevin McDonald as Albus Duckweed
- James Patrick Stuart as One-Eyed Wally
- Troy Baker as Captain Grime
- Laila Berzins as Sadie Croaker
- Brian Maillard as Leopold Loggle
- Kevin Michael Richardson as Mr. Flour
- Jill Bartlett as Maddie Flour
- Dee Bradley Baker as Bessie
- Kaitlyn Robrock as Felicia Sundew
- Katie Crown as Ivy Sundew
- Mona Marshall as Sylvia Sundew
- John DiMaggio as Stumpy
- Michelle Dockery as Lady Olivia
- Keith David as King Andrias
- Marlow Barkley as Rosemary Flour
- Mia Allan as Lavender Flour
- Ella Allan as Ginger Flour
- Zehra Fazal as General Yunan
- Tony Hale as Apothecary Gary and Lloyd
- Chris Sullivan as Gunther and Teddy
- Tara Lipinski and Johnny Weir as talent judges
- Diedrich Bader as Frog Jordan
- Paul Eiding as Monroe
- Keith Silverstein as Barry
- Kermit the Frog (Matt Vogel) as Crumpet the Frog
- Jenifer Lewis as Mama Hasselback
- Alex Hirsch as The Curator and Frog Soos
- George Takei as Mr. Littlepot
- Kristen Schaal as Bella the Bellhop
- Hugh Bonneville as Wigbert
- Tress MacNeille as Doris
- Susan Egan as Renee Frodgers
- Misty Lee as Priscilla "the Killa" Paddock
- Romi Dames as Pearl Paddock
- Flula Borg as Professor Herringbone
- Whoopi Goldberg
- RuPaul as Mr. X
- Kate Micucci as Terri
- Brad Garrett as Robert Otto
- Cassie Glow as Molly Jo
- Anika Noni Rose as Dr. Jan
- Archie Yates
- Wallace Shawn as Humphrey Westwood
- Wayne Knight as Ned
- Jason Ritter
- Dana Davis as Jess
- Melissa Villaseñor as Ally
- Cree Summer as Dr. Frakes
- This is the first time Brenda Song has starred in a Disney Channel production since the ending of The Suite Life of Zack & Cody sequel, The Suite Life on Deck in 2011.
- Amphibia is the first animated series in history to center on a Thai-American character.
- The episode title cards depict a wood carving in darkness lit up with the title superimposed over it. This is an actual wood carving that is photographed and reused every episode.
- The show was originally titled Amphibiland, but was changed due to sounding too similar to Amphibilands, the setting of the young adult novel series An Army of Frogs which was adapted into the animated series Kulipari, which was released on Netflix from 2016 to 2018. Both series also have Keith David voicing a main villain.
- This is the second animated Disney production in which Anika Noni Rose, Keith David, and Jenifer Lewis appear, after The Princess and the Frog. Ironically, frogs were the main theme in that production, in which David also voiced the main villain.
- Rebecca Sugar, of Steven Universe fame, will write a song for season three's Christmas special. This marks the first time Sugar, who had primarily worked over at Warner Bros., will be involved in a Disney production. Braly previously worked on the Steven Universe episode "Lars and the Cool Kids".
- "“AMPHIBIA” SEASON 3 COMING SOON TO DISNEY CHANNEL & DISNEY+ THIS FALL!". What's On Disney+? (July 24, 2021).