Ena appears when she is with Bambi's mother out in the meadow when Bambi hides behind his mother after being chased by Faline. Faline tells Ena that Bambi is bashful, to which Ena replies that he may not be if Faline says hello to him. Bambi later chases Faline around Ena and his mother before they run further into the meadow together whilst being looked on by their mothers.
- In the original novel on which the film is based on, Ena is actually Bambi's maternal aunt.
- In the book, she's the mate of an unknown buck, and also the mother of Faline's twin brother, Gobo, who would later fall in love and become mates with a young doe named Marena, who, as a result, becomes her daughter-in-law.
- In the book's sequel, Bambi's Children, she gains two adoptive grandchildren when Faline adopts the two orphaned bucks, Mambo and Nello.