Aunt May was created by Stan Lee and Steve Ditko.
In Civil War, she is visited by Tony Stark, who is looking to recruit Peter under the pretense of a grant. In a post-credits scene, she tends to Peter's injuries, unaware of his dual identity.
Aunt May also appears in Spider-Man: Homecoming, where she acts as a major source in Peter's life. She is concerned about the crimes Spider-Man foils being close to their home. She comforts him after he loses his "Stark Internship" and later helps him when he acquires a date for the homecoming dance. At the end of the film, she finds Peter wearing his Spider-Man suit and reacts in shock.
Though she does not appear in Avengers: Infinity War, the events of Spider-Man: Far From Home reveal that she was eliminated by Thanos's snap.
In Avengers: Endgame, May is resurrected by Professor Hulk alongside the victims of Thanos's snap. She attends Tony Stark's funeral alongside her resurrected nephew.
In Spider-Man: Far From Home, she not only is aware that her nephew is Spider-Man but also supports him as his alter-ego. May is hosting a charity event for the homeless that were dusted after Thanos' snap, along with Peter disguised as Spider-Man. May is later seen encouraging Peter to take his Spider-Man suit with him him to Europe and puts it in his suitcase after he refuses. During the time that Peter is away on his school trip, Aunt May gets closer to Happy Hogan, who is helping her out at the shelter. May is later seen speaking with Peter about his plan to win Michelle Jones's heart, clearly aware about her nephew's crush. May (in a deleted scene) comes to the airport a week later, after the school trip ends, to pick her nephew up. Back at their apartment, Peter asks May and Happy if they are dating and while Happy says they are, May denies it saying it wasn't a serious relationship but more of a fling.
Aunt May will return in Spider-Man's third standalone film and be dealing with her nephew's identity having been exposed to the world.
Marvel Animated Universe
Aunt May is a recurring character in the Ultimate Spider-Man TV series, voiced by Misty Lee.
Like her Ultimate comic book counterpart, this version of Aunt May is considerably younger and less frail than her traditional portrayal. She is shown to be involved in various hobbies such as yoga and cooking classes, which allowed Peter to sneak out of the house without arousing suspicion. In "Attack of the Beetle", she was asked out to dinner by S.H.I.E.L.D. Agent Phil Coulson (who Nick Fury assigned to be the acting principal of Peter's school). Near the end of Season 3, she reveals she has always known that Peter was Spider-Man, but despite her knowledge, she thinks that nothing has changed between them.
Though she does not appear in this crossover, Spider-Man mentions her in the title sequence by complaining, "Aunt May, Phineas and Ferb are making a crossover!"
- The Marvel Cinematic Universe version of May is the third portrayal of the character in Marvel's cinematic history. May was previously portrayed by Rosemary Harris and Sally Field in Sony's two Spider-Man film series. She was also voiced by Lily Tomlin in the animated film Spider-Man: Into the Spider-Verse.
- Marisa Tomei has previously acted alongside Robert Downey Jr. in the films Only You and Chaplin. This may possibly be the reason why Tony Stark is attracted to her, as a call back to the former.
- Tomei's casting, at her early 50s, was criticized as fans believed she was too young and attractive to play Aunt May. Kevin Feige has stated that being in her 50s makes her realistically old enough to be Peter's aunt, as most of her incarnations are depicted as elderly.
- Nancy Linari reprises her role as Aunt May in the non-Disney related 2018 Spider-Man video game.
- Spider-Man: Homecoming marks the first time in a Spider-Man film that Aunt May finds out Peter Parker is Spider-Man.
- Originally, after the release of Avengers: Infinity War, directors Joe and Anthony Russo claimed that May survived Thanos's snap. However, Spider-Man: Far From Home instead reveals that May was disintegrated by Thanos.
- The casting crew for Captain America: Civil War wanted Aunt May to be younger as they didn't think it was right for Peter Parker to have an elderly aunt when he was just a teenager.
- Although she and Been are not physically seen, May can be heard in Iron Man 2 during the Stark Expo battle. Not long before Iron Man saves a young Peter Parker, May and Ben are heard shouting Peter's name. While it is possible the people shouting his name could be his parents, both of Peter's parents have been known to have died before 2010, and Iron Man 2 takes place in 2009.