Aunt May was created by Stan Lee and Steve Ditko.
In Civil War, she is visited by Tony Stark, who is looking to recruit Peter under the pretense of a grant. In a post-credits scene, she tends to Peter's injuries, unaware of his dual identity.
Aunt May also appears in Spider-Man: Homecoming, where she acts as a major source in Peter's life. She is concerned about the crimes Spider-Man foils being close to their home. She comforts him after he loses his "Stark Internship" and later helps him when he acquires a date for the homecoming dance. At the end of the film, she finds Peter wearing his Spider-Man suit and reacts in shock.
Though she does not appear in Avengers: Infinity War, the events of Spider-Man: Far From Home reveal that she was eliminated by Thanos's snap.
In Avengers: Endgame, May is resurrected by Professor Hulk alongside the victims of Thanos's snap. She attends Tony Stark's funeral alongside her resurrected nephew.
In Spider-Man: Far From Home, she not only is aware that her nephew is Spider-Man, but also supports him as his alter-ego.
Marvel Animated Universe
Aunt May is a recurring character in the Ultimate Spider-Man TV series, voiced by Misty Lee.
Like her Ultimate comic book counterpart, this version of Aunt May is considerably younger and less frail than her traditional portrayal. She is shown to be involved in various hobbies such as yoga and cooking classes, which allow Peter to sneak out of the house without arousing suspicion. In "Attack of the Beetle", she was asked out to dinner by S.H.I.E.L.D. Agent Phil Coulson (who Nick Fury assigned to be the acting principal of Peter's school). Near the end of Season 3, she reveals she has always known that Peter was Spider-Man, but despite her knowing, she thinks that nothing has changed between them.
Though she does not appear in this crossover, Spider-Man mentions her in the title sequence by complaining, "Aunt May, Phineas and Ferb are making a crossover!"
- The Marvel Cinematic Universe version of May is the third portrayal of the character in Marvel's cinematic history. May was previously portrayed by Rosemary Harris and Sally Field in Sony's two Spider-Man film series. She was also voiced by Lily Tomlin in the animated film Spider-Man: Into the Spider-Verse.
- Marisa Tomei has previously acted alongside Robert Downey Jr. in the films Only You and Chaplin. This may possibly be the reason why Tony Stark is attracted to her, as a call back to the former.
- Tomei's casting, at her early 50s, was criticized as fans believed she was too young and attractive to play Aunt May. Kevin Feige has stated that being in her 50s makes her realistically old enough to be Peter's aunt, as most of her incarnations are depicted as elderly.
- Nancy Linari reprises her role as Aunt May in the non-Disney related 2018 Spider-Man video game.
- Spider-Man: Homecoming marks the first time in a Spider-Man film that Aunt May finds out Peter Parker is Spider-Man.
- Originally, after the release of Avengers: Infinity War, directors Joe and Anthony Russo claimed that May survived Thanos's snap. However, Spider-Man: Far From Home instead reveals that May was disintegrated by Thanos.