Experiment 040 was the 40th genetic experiment created by Jumba with Hämsterviel's funding. He was designed to dig up vegetation. 040 and the other first 624 experiments were deactivated and smuggled to Earth by Jumba during his mission to capture Experiment 626.
All of the experiment pods were released and scattered across the island of Kauai.
In "Remmy", Backhoe was one of the experiments in Lilo's dream.
Backhoe was one of the experiments that were gliding on the screen, but he is 569 instead of 040.
The first 624 experiments, including Backhoe, were rounded up by Leroy and taken to a stadium to be destroyed. However, Lilo, Stitch, Jumba, Pleakley, Reuben, and Gantu arrived before the experiments could be destroyed.
Backhoe participated in the following battle between the experiments and the Leroy clones.
The Leroys soon gained the upper hand in the battle but were defeated when Lilo, Stitch, Reuben and several other experiments performed the song "Aloha ʻOe", which caused the Leroy army to shut down due to the original Leroy's fail-safe.