Barry and Bob are Texas longhorns who both talk in Southern drawls and are somewhat slow-witted. Like the other bulls on the drive, they seldom see cows and become easily attracted to any in the vicinity. They also apparently have a crush on Pearl's dairy cows, as they pursue the trio in a very insistent manner.
Barry and Bob are first seen sleeping at a cattle drive with the other 4,998 cattle. When Maggie, Grace, and Mrs. Calloway pass by, they all wake up and Barry and Bob begin to flirt with the dairy cows, much to Maggie and Mrs. Calloway's disgust, though Grace doesn't seem to mind the bulls' company. She then says that Barry and Bob might be able to help them, to which the bulls suggestively agree (in a flirtatious tone) that they can all "help" each other. Knowing what Barry and Bob meant, Maggie and Mrs. Calloway become even more disgusted.
When Alameda Slim and the Willie Brothers arrive and knock out the owners of Barry and Bob, Grace takes notice and declares it's Slim and the Willies. Barry and Bob panic upon hearing this and offer to be the cows' bodyguards. Mrs. Calloway then knocks Barry out with her hoof in annoyance when he (mistaking her for the "blonde") puts his hoof on her in an unwelcome way. Barry and Bob, along with all the other cattle, are later hypnotized by Slim's yodeling song and led away.
Barry and Bob are seen again when Pearl's cows, along with Buck and Lucky Jack, hijack a train to reach Little Patch of Heaven and prevent it from being sold by Slim in his Yancy O'Del disguise. As the bulls chase after the cows, Maggie and Mrs. Calloway tell them off, explaining that they have a farm to save. Barry and Bob once again offer to "help" the cows on their mission, but they both bump into a U.S. mail pole, knocking themselves out. Grace then invitingly tells the bulls to come up and see them sometime.
Barry and Bob are last seen on Little Patch of Heaven with Junior the Buffalo after Slim is arrested and Pearl's farm is saved. The bulls and Junior express their intimacy using poker terms, to which the three cows chuckle and let them all come in. Barry and Bob end up courting Maggie and Grace, while Junior courts Mrs. Calloway. They also dance with all the other farm animals as they celebrate Little Patch of Heaven being salvaged and re-opened.