For Disney, Trejo filmed a cameo for the 2011 film, The Muppets as a prisoner; however, his scene was cut from the final theatrical film. Footage from the scene was shown in The Fuzzy Pack trailer. He did, however, have a role in Muppets Most Wanted, where he plays a Gulag prisoner (also named Danny Trejo; Trejo was an inmate at San Quentin State Prison for 3 years).
His other Disney roles include Johnny-23 in the 1997 Touchstone Pictures film, Con Air, Venom in Phineas and Ferb: Mission Marvel, Antonio Agama in Elena of Avalor, Wreck and Malice Marauder in Tangled: The Series, Vasquez in Big City Greens, Slim in Bubble Boy, Pierce in Six Days, Seven Nights and the lead piñata in the Mickey Mouse episode "¡Feliz Cumpleaños!" and Cousin Huet in Muppets Haunted Mansion.