- Mickey Mouse
- Minnie Mouse (Hot Dog Dance only)
- Daisy Duck (Hot Dog Dance only)
- Donald Duck
- Toodles (does not speak)
- Willie the Giant (debut)
- Boo-Boo Chicken (debut)
- A bubble machine
- A magnifying glass
- A teddy bear
- Title: Donald and the Beanstalk - The title is a play on the story, Jack and the Beanstalk.
- This is the only Mickey Mouse Clubhouse episode to date to have five Mouseketools instead of four.
- Just as in the original Mickey and the Beanstalk film, Donald Duck angrily throws the magic beans to trigger the sprouting of the beanstalk.
- This is the second time Mickey had to be quiet during the Mouseketools segment after “Mickey Goes Fishing”.
- This is the first episode to feature Willie the Giant and Boo-Boo Chicken.
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