Though he is not an actual villain, but merely an actor, Bolt thinks he is really a villain and promises to get him for real.
Dr. Calico is among the most ruthless, cunning, and merciless villains ever seen in the Disney universe. Always accompanied by one or more cats, he is a master of planning and a brilliant strategist. Ruthless, yet patient, he likes to torture his enemies in order to force them to do his bidding. Although he is seen always hiding behind his guards, he is not above killing his enemies with his bare hands; this is seen in the show when he tries to kill Penny after she had surgery by attempting to inject her with some sort of fatal drug. In the video game, his personality seems more evil and sinister than in the film. Also in the video game, he also has a tendency to infuse himself with lightning so he can fight Bolt, though it is unknown if he uses it temporarily.
In reality, Dr. Calico (real name unknown) is an actor, and really just a normal and well-intentioned man. Although he isn't a villain in real life, it is unknown if he was aware of Bolt believing him to be a real villain nor his escape because of the Director's actions.
Dr. Calico is first seen on video with Penny's father captive, talking to one of his associates as Penny and Bolt spy on him. As the associate tries to leave, Penny and Bolt capture and interrogate him, learning of Calico's base in Bolivia. Calico then has his military forces mobilized, leading to a high octane chase around the city.
Later, the cats portraying Calico's cats sneak into Bolt's trailer and mock him, mentioning that Calico has a plan and that he will execute that plan and then execute Penny.
Calico does not appear in person until the next day's shooting, where he appears with his cats and confronts Penny and Bolt (who have infiltrated his Bolivia base). He imprisons Penny in what appears to be a portable toilet and flies away, laughing maniacally.
Calico is mentioned several times throughout the film. When Bolt attempts to break free, he spots the prop of the toilet Calico used to abduct Penny and tries to smash his head through the window to access it but fails. Also, when he arrives in New York by mistake, he notices a man who looks like Calico from the back. Bolt tries to knock the man unconscious, but all Bolt does is simply tap the man on the shoulder. Following this, Bolt notices a truck containing a portable toilet resembling Calico's, proceeding to intercept the truck.
After meeting up with some street pigeons, Bolt mentions that he is tracking down Calico. The pigeons lead him to Mittens, since Calico has a fondness for cats. Bolt then interrogates Mittens, demanding that she tell him where Calico and Penny are.
After Rhino and Bolt break free from the animal control truck, Rhino motivates Bolt using several of Calico's plots as an example.
When Bolt arrives in Hollywood, Calico is seen once more, showing a captive Penny to her father when the scientist refuses to give up the research. She calls for Bolt, and his lookalike arrives to rescue her, much to Calico's shock as he shrieks, "GET THAT DOG!!!" Unfortunately, Bolt's lookalike is spooked by Calico and his minions and flees the set, setting fire to the area and forcing Calico and the crew to leave.
Calico is revealed to have escaped the studio as he, while disguised as a medic at the hospital in the TV show, sneaks up on a new Penny with a possibly deadly drug. However, a new Bolt destroys the syringe and rescues Penny, but they are abducted by aliens as Calico looks up and clenches his fist in anger at the aliens.
Calico returns in the short as the main antagonist. Here, he captures Bolt and Penny, preparing to dip the both of them in lava. Unknown to Calico, Penny's father overhears Calico gloating over his dome-shaped base and defenses through Bolt's collar, prompting the scientist to infuse Rhino with Bolt's powers and send him to rescue Bolt and Penny.
Rhino arrives at the base and proceeds to smash his way through Calico's defenses, eventually using his laser vision to break into the dome. After a brief conversation with the enhanced hamster, Calico unleashes a barrage of missiles and artillery, but Rhino hops on one of the missiles. As the missile trails onward, Rhino destroys the rest of the artillery and rescues Bolt and Penny, sending the missile towards Calico. He rises from the rubble and swears revenge, but Rhino promptly finishes Calico off with a super-squeak, leaving a defeated, but singed Calico confused.
In another dream sequence, Calico is mentioned by Rhino when he tells the President of the U.S. about the former's evil plot to destroy the moon with a huge laser.
Dr. Calico is the main antagonist of the video game, which is inspired by the film's in-universe TV show. As a result, he is more fleshed out as a character.
After capturing Penny's father on the show, Calico then pursued the one thing that would get him to cooperate, Penny. Calico finally managed to capture Penny once she and Bolt found his temple base in the Yucatán jungle. To prove Penny was alive and in the custody to her father, Calico gave Penny a cell phone with her father on the other line. Calico then tried to flee the crumbling temple with his new captive but decided to leave her there with Bolt to die, saying, "As long as the professor believes she's alive, he will do what is asked and finish the weapon. Too bad we can't all have nine lives."
However, Penny and Bolt survived and discovered Calico's new base in Northern Russia, containing a large rocket. But upon entering, Penny notices micro-focusing mirrors and realizes that it is her father's satellite instead of a warhead. All too late as Calico seals the entrance and, to Bolt's horror, launches it with Penny inside. Bolt then jumped onto the rocket and dismantled it in mid-flight, crashing it into the snow.
The two then ventured to Calico's harbor in China, where they discovered a ship full warheads. Just then, Calico arrived at the harbor, enraged and surprised to find Penny still alive, and even more so as he watched his prized freight ship sink into the water, thanks to Bolt, who then pursues Calico, escaping in his car to a nearby train station. He arrives at the station and boards his train, closely followed by Penny and her dog. Just as the duo finally corners the doctor, he slips into a hidden room and escaped on his private jet, but not before Penny secures a homing beacon onto the hull, leading them right back to Calico's base.
Penny and Bolt traced his jet back to his main base, where Penny's father was being held. However, upon arriving, Calico used his new weapon on Bolt, draining him of his powers and strength and capturing Penny. Once Penny is inside, he explains his plan to use Penny's father's micro-focusing mirror-equipped satellites to bounce a beam around the Earth and neutralize and destroy all atomic weaponry except for his own. And as he leaves, he violently kicks Bolt's cage proving Penny of her limited knowledge. However, during this, Penny maneuvered her way out of her hand bindings.
Once free, she attempted to find her father to save Bolt. Unfortunately, she is only captured by the doctor once again, just as Bolt regains his powers and Penny sabotages the control panel, however, raising the bridge so Bolt could reach her, infuriating Calico. Once he did, Penny escaped from her guard captors yet again and launched a prepared, rocket powered cart at the main power generator (which Bolt exposed). Calico then infused himself with the remaining power and dueled with Bolt in a hard-fought lengthy battle, but was ultimately defeated. Beaten and weakened, he attempted to escape on his private helicopter, launching numerous missiles as a decoy, just as Penny finally finds her father (aboard the helicopter). But while Bolt tried to apprehend Calico by grabbing his foot, Penny's father pleads to her and Bolt to allow Calico to escape with himself to stop the missiles or innocent people would die, reminding her that she had the power to find him again. Regretfully, a disappointed Penny ordered Bolt to stop the missiles as Calico escaped with her father.
- Dr. Calico is one of the few Disney Villains who is not evil due to the fact that he is simply an actor playing a villain in the Bolt TV show. This also makes him the first Disney Villain to not have any genuine hatred towards or a vendetta against the film's heroes, with the second being the Backson.
- Calico is also the first character to be simply an actor playing a villain but mistaken by at least one character to be an actual villain, with the second being Wreck-It Ralph who is just a villain who wants to be a hero until redeeming himself at the end.
- Despite not being an actual villain, Dr. Calico still drives the plot of the film.
- Although he is called Dr. Calico, it is never really explained what kind of doctor he may be. It is implied that aside from being the leader of a major terrorist organization, he could also be a medical doctor or a scientist of genetics and / or robotics.
- According to Rhino, Calico once had a plan to infiltrate the Olympics with gymnastic cyborgs, which was foiled by Bolt in an episode that wasn't shown in the film.
- Calico's appearance and personality are modeled after those of some of James Bond's most famous villains such as Dr. No, Auric Goldfinger, Ernst Stavro Blofeld, Aristotle Kristatos, Hugo Drax, Brad Whitaker and Francisco Scaramanga.
- Dr. Calico is one of the few villains to not have his own leitmotif or musical number, unlike such villains as Captain Hook, Jafar, Scar, Judge Claude Frollo, Oogie Boogie, Ursula, and Dr. Facilier. This could be possibly be attributed to the fact that he is not a true villain and that the above characters are all featured in musicals, whereas Bolt is not a musical.
- It is unknown if Calico's cat "eye" is in fact real. It is heavily implied that his eye may be just a fake green eye used as a patch.
- Calico's in-universe actor's name is unknown.
- Dr. Calico's plan to capture a scientist and then get him to work for him by threatening his daughter is also Professor Ratigan's plan in The Great Mouse Detective, where the inventor is Hiram Flaversham and the threatened daughter is Olivia Flaversham.
- Dr. Calico is also somewhat similar to other evil Disney mad scientists who scheme for world domination, such as Dr. Drakken from Kim Possible. Drakken, however, doesn't have cats as part of his world domination schemes.
- His main phrase is "GET THAT DOG!", though it is unknown if its his catchphrase as he only says it once in the film along with its trailer.