In the animated film industry she was best known as the voices of the evil stepmother Lady Tremaine in the Disney animated film Cinderella and the wicked fairy Maleficent in Disney's Sleeping Beauty. For both films, animator Marc Davis created the characters' facial features to resemble Audley.
She also provided the voice of Madame Leota in the Haunted Mansion attractions in Disneyland and Walt Disney World, speaking the memorable lines, "Rap on a table. It's time to respond. Send me a message from somewhere beyond!"
- Due to voicing Lady Tremaine and Maleficent, Eleanor Audley is the one of the two classic Disney Princess Villain actresses, the other one being Lucille La Verne, who voiced the Evil Queen.
- Interestingly, both Lucille and Eleanor were born in November. However, Eleanor's birthday is November 19, while Lucille's birthday is November 8.
- Eleanor Audley also appeared alongside Eva Gabor in the 1965 TV show Green Acres.