Ellie Mae is first seen in her house at Devil's Bayou with her husband Luke , she is watching the sky and notices that her friend Orville is burning and urges Luke to wake up. They watch as Orville fall on a nearby pond with two passengers Bernard and Miss Bianca, she quickly rushes to help Miss Bianca while Luke helps Bernard. They inform them that they are from New York, soon they hear Medusa in her swampmbile and Ellie Mae remarks that she would like kick her big head, then they see that Penny has been captured by Medusa's pets Brutus and Nero. Ellie Mae mentions with sadness "that poor creature tried to ecape again" which means that the Swamp Folks know about Penny's capture. Bernard and Bianca decide to follow Brutus and Nero, Ellie Mae gets them the boat of Evinrude, she tells the mice that she will gather all the neighboors for help.
Later in the film Ellie Mae has gathered all the neighboors in her house: Deadeye, Gramps, Gopher and Deacon Owl; they are waiting for Evinrude's signal for attack. Evinrude arrives to Ellie Mae and Luke's house exausted after being chased by hungry bats, Luke gives him a drop of his moonshine and Evinrude regains his energy and calls everyone to attack. All of the Swamp Folks race to the riverboat where Medusa is holding Penny and Mr. Snoops.
Soon a fight starts and all of the Swamp Folks start attacking Medusa, Ellie Mae uses a rolling pin as a weapon against Medusa. The villain manges to break free and starts shooting at the animals who encounter with Brutus and Nero, making the Swamp Folks run away with Ellie Mae going to the roof of the boat. Medusa starts shooting at Penny and when she is about to deliver a lethal shot at the girl, Ellie Mae jumps on her head and knocks her with her rolling pin (finally making her wish come true of kicking Medusa in the head). Penny and all of the animals plan to flee on the swampmobile but Bernard notices that there is no gas, so Ellie Mae makes Luke fill the gas tank with his moonshine. As they manage to start the car, Medusa jumps on them managing to get a grab on the swampmbile, but Ellie Mae hits her in the hands making Medusa loose her balance but the villain grabs to a rope and chases them making water skii with her pets but the rope is cut and Medusa is sent flying through the airs. The group escapes and Ellie Mae happily coments that they give Medusa what she deserved.
Ellie Mae is not seen for the film's end, but it can be assumed that Bernard and Bianca told them that Penny had been adopted.