Gamora is a leading member of the Guardians of the Galaxy alongside Star-Lord, Drax the Destroyer, Rocket Raccoon, Groot, and Nova. The Guardians battle the Chitauri an alien race led by Korvac. When Rocket Raccoon's ship is pulled into the Chitauri ship, Rocket fakes surrender so that they can free the captive Guardians of the Galaxy members. Afterwards, Spider-Man discovers that the Chitauri are planning to destroy the Earth. The Guardians plan to run the Chitauri ship into the sun while fighting the Chitauri forces. When their tactic does not work, the Guardians end up making their way towards the Dark Matter Cannon. While Nova and Spider-Man head to the Dark Matter Cannon, the Guardians fight the Chitauri and Korvac. After Korvac is defeated by Groot, Nova destroys the Dark Matter Cannon and the Chitauri ship is destroyed.
In the episode "The Return of the Guardians of the Galaxy", Gamora was seen recuperating on the Guardians of the Galaxy's spaceship when they land on Earth at the time when Titus leads the Chitauri into targeting Nova's helmet. When she recovers in time to help fight Titus' forces, she voiced her disdain that they had to land on Earth. After Titus was defeated and the remaining Chitauri escape, Nick Fury was displeased that Gamora showed up on Earth again when she said that she wouldn't return. Gamora stated "I lied" and then left with the Guardians of the Galaxy with Titus in their custody.
