The den first appears when Janja berates Cheezi and Chungu for letting Bunga go after he was saved by Kion via The Roar of the Elders. Just then, a vulture named Mzingo arrives and informs Janja of Kion assembling a new Lion Guard to protect the Pride Lands.
The den is a recurring place in the series. But it is very rarely at the center of attention.
Mzingo flies to the den to inform Janja that Kiara has been made temporary queen and is squabbling with Kion. This gives Janja an idea to take advantage of Kiara's inexperience and take over the Pride Lands.
Upon learning that he is immune to snake bites, Bunga believes that he is invincible and goes to the Outlands and attempts to climb the den's volcano, but is confronted by Janja and his clan. Luckily, Bunga is saved just in time by Fuli.
Janja finds two baboons named Mwevi and Mwizi in his den and chases after them.
Ushari uses the volcano to revive Scar.
Scar is destroyed and Ushari is thrown in the lava.