Ma Tembo is seen at her father's funeral with the rest of her herd. She watches the other elephants lay their tributes, and smiles when a baby elephant lays their flowers down upon the corpse of her father. Later, she announces that Simba will be saying a few words about her father. She listens to Simba's mistake in speaking elephantese and is able to recall fond memories of her father.
Ma Tembo and her herd hold a concert at Mizimu Grove, which is attended by Nala and Kiara (as it is traditional for the royal family to attend), after Kion had to go and help antelope stuck in the mud and Simba was trapped in a sinkhole. Just as the concert ends, Simba, Kion, Timon, Pumbaa and The Lion Guard arrive. Simba apologies to Ma Tembo and the other elephants for being late and to make up for it sing "Hakuna Matata" with his family and the Lion Guard.
Ma Tembo is seen heading towards Pride Rock for the Savannah Summit with several other leaders, including Makuu. She is one of the few animals that accepts Makuu being at the event. She then heads to Mizimu Grove, eventually getting comfortable and snuggling up to everyone during the song, Welcome to the Summit. In an effort to find out who's plotting against Makuu, Kion convincingly tells the leaders that Makuu completed his journey through the Circle of Life, with Ma Tembo expressing sadness. When Makuu is revealed to be alive and well and forgiving towards those who wronged him, she lets out a large cheer as the summit continues.
One morning, the Lion Guard notice several animals running off in a certain direction. When Ma Tembo rushes past the Lion Guard, they try to find out what she's in a hurry for, but she tells them that she can't stop. She makes her way to a sausage tree, having heard of a marvelous show from other animals. When the performers arrive, Uroho, the leader of The Traveling Baboon Show, performs a magic trick with her, with shocks the elephant. After enjoying the show, she and most of the animals leave the area.
But when they discover a large patch of land devoid of food, she calls for the Lion Guard to help assist her. With the food disappearing so suddenly, she is certain that there's a food thief, and the Lion Guard agree to help. Ma Tembo then guides the other animals away to find more food, leaving the Guard to their work. A bit later, after being discovered as the thieves, Uroho offers Ma Tembo a fruit which she graciously accepts. Feeling bad, the baboons offer her and the other animals a free show, as their way of apology.
"The Rise of Scar"
Ma Tembo and her herd are searching for the new water source for the Dry Season. Janja's clan starts to chase them but The Lion Guard comes to save them. Later the hyenas and the baboons are chasing her herd and Kion uses his roar to blow the hyenas away. After hearing about what's going Kiara asks Simba if she could help her. Soon Makini, Rafiki's new apprentice asks if she could come too. Kiara and Makini go and help Ma Tembo. Makini says that everyone needs to be quite so Ma Tembo can find the water. Ma Tembo soon finds it after everyone helps her dig it out and Kion congrats her
Rafiki's New Neighbors
Kion and Bunga bring Chama a member of herd back to her herd after hearing that he was kicked out. Ma Tembo said it was for a good reason and that he was an disruption to them. However she welcomes him back if he promises to behave himself. Chama didn't feel good their so he just left them.
The Ukumbusho Tradition
Ma Tembo's herd is getting ready when the Lion Guard and Makini come. Makini gets excited to paint the sunburst on the elephants. Ma Tembo wants everything to be perfect since this is King Simba's first ever Ukumbusho. Timon and Pumbaa come to help the Lion Guard learn their lines. When Ma Tembo didn't like how the guard wasn't all lions Makini has an idea to make them look like lions. Later when Makini runs out of paint for the elephants she uses flowers which attracted bees. Bees come and the elephants panic however the guard saves them. Makini apologies and Simba thinks that they should have a new tradition and show goes back on.
Timon and Pumbaa's Christmas
Ma Tembo's herd helps perform "The Twelve Ways Of Christmas" for Timon and Pumbaa.
The Kilo Valley Fire
Ma Tembo and her herd home gets destroyed by a fire from Scar. Kion tries to find them a new home but when no one wants them they try to move away. Soon Laini's home is on fire and the elephants come to help. Laini welcomes the elephants to come and stay with her and the galagos.