Madame Upanova is a black ostrich with feminine features. She always loves pink as her favorite color, so she wears a pink bow with matching ballet slippers and has a thin black ribbon around her neck.
Madame Upanova is in a segment called "Dance of the Hours". She appears in the beginning with all her students. She wakes herself and all of them up as the curtains open. Her pirouette is caused by their reach over her as it is thrust up high with lots of feathers shed. As she feeds some of her students some fruit from the conch, she tries to eat the purple grapes she holds in her beak and get away from the squabbling of all her students. As they pursue her, one of them makes her drop the grapes, causing them to fall into the water, and they all flee from the bubbles coming from the fountain.
Madame Upanova makes two cameo appearances in Who Framed Roger Rabbit. First, she walks to the Maroon Cartoons Studio door, giving Eddie Valiant the cold shoulder as she passes him. Her second appearance is among a group of toons in the finale.
Madame Upanova makes a cameo in one of the episodes conjured by Genie, where she is shown holding an egg.
Her role is unknown due to this program being canceled.
Ostriches resembling Madame Upanova (possibly her students in her colors) are walkaround characters that appear in parades. Upanova also appears in the Disneyland Hotel's Fantasia Store as a statue. A statue also appears outside the pool area at the Disneyland Hotel at Disneyland Paris. She can also be seen in the Main Street Electrical Parade and in Fantasmic!'s bubble sequence.
- Despite being referred to as female by the development staff, Madame Upanova's feather colors are of a male's. Much like peacocks where males are bright and colorful, and females are shades of brown and grey, only male ostriches have black and white feathers, while females are varying shades of brown like female peacocks.
- In production, Madame Upanova was referred to as Mademoiselle Upanova; however, she is marketed as Madame Upanova.