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Mickey conducts an opera starring Donald Duck and Clara Cluck, and Pluto steals the show.
- Mickey Mouse (voiced by Walt Disney)
- Donald Duck (voiced by Clarence Nash)
- Pluto (voiced by Pinto Colvig)
- Clarabelle Cow
- Clara Cluck (voiced by Florence Gill)
- Horace Horsecollar
- On the Limited Gold Edition: Mickey VHS, this cartoon uses the RKO opening and closing titles from Symphony Hour, and the title card is completely absent. The original opening and closing titles were restored on the Walt Disney Treasures Mickey Mouse in Living Color Volume 1 DVD and the Walt Disney Classic Cartoon Favorites, Volume 6: Extreme Music Fun DVD.
- The recording session of this cartoon was reenacted by Florence Gill and Clarence Nash in The Reluctant Dragon.
- Walt Disney Cartoon Classics: Limited Gold Editions: Mickey (1952 reissue print)
- Mickey and Company
- Walt Disney Treasures: Mickey Mouse in Living Color
- Walt Disney's Classic Cartoon Favorites: Extreme Music Fun