Unlike the previous Mickey Mouse Clubhouse, Minnie's Bow-Toons is not interactive.
Minnie Mouse and her best friend Daisy Duck are showing their love of fashion by opening up their very own Bow-Tique, which will sell bows and bow ties. This new series also showcases Millie and Melody, Minnie's nieces.
- Russi Taylor - Minnie Mouse
- Tress MacNeille - Daisy Duck
- April Winchell - Clarabelle Cow
- Frank Welker - Figaro
- Nika Futterman - Cuckoo Loca
- Avalon Robbins - Millie and Melody
- Bill Farmer - Goofy and Pluto
- Bret Iwan - Mickey Mouse
- Tony Anselmo - Donald Duck
- The boys do not appear in this series, but Mickey appeared in "Tricky Treats", "Oh, Christmas Tree!" and "Turkey Time", Donald appeared in "Oh, Christmas Tree!" and "Turkey Time", and Goofy appeared in "Dance Lessons", "A Good Sign", "Oh, Christmas Tree!" and "Turkey Time". There is also a heart shaped picture of them in the bedroom on the wall between Minnie and Daisy's beds.