- “For generations, the Tomorrowland Transit Authority has worked to connect the universe. But now, a dangerous power is rising. To stop it, the TTA has put together a team of extraordinary heroes. They are Mission Force One.”
- ―Season 3 introduction
Mission Force One is a team of kids and their robot-pets led by Miles Callisto, who appear in the Disney Junior animated series Miles from Tomorrowland. Formed by Commander Copernicus, head of the TTA, Mission Force One is to connect and protect the Tomorrowland Transit Authority against a new force, Commander Nemex of the Nemesystems, who wishes to conquer the universe. Mission Force One uses STEAM (Science, Technology, Engineering, Arts, and Math) to complete their missions. They travel on the starship Zenith, which is captained by Miles.
- Miles Callisto: Miles is the team leader and commanding officer aboard the Zenith, and represents Science. Chosen as team leader because of his courage and leadership whenever there is a problematic situation. Miles wears an armored red and black mission suit fitted with many gadgets and weapons. His choice of weapons are his Lazerang which can become a Lazerstaff and a blast shield. He also has super speed. Miles's robot-pet is a robo-ostrich named Merc.
- Loretta Callisto: Loretta serves as the team's mission specialist, and represents Technology. She wears a pink and black suit, armed with chakram projectors that deploy unlimited holo-chakrams from her gloves. Loretta's robot-pet is a robo-monkey named Miss baker.
- Mirandos Ariellian: Mirandos is the engineer member of the team and represents Engineering. She wears a blue and black suit with a cloak. She wears Solidizer gloves that gives her the ability to create any kind of object to use on a mission. Mirandos's robot-pet is robo-penguin named Bowtie.
- Haruna Kitumba: Haruna is the navigator aboard the Zenith and the muscle member of the team, and represents Math. He wears a yellow and black armored suit equipped with gloves that allow him to punch through anything. Haruna's Robot-pet is a robo-dog named Sly.
- Blodger Blopp: Blodger is ship operator aboard the Zenith, and represents art. His suit gives him the ability to shapeshift into any inanimate object he can think of. Blodger's robot-pet is a robo-tortoise named Sheldon.