The twins first appeared in Mickey's Nephews on September 18, 1932, introduced by Gottfredson as the children of the nanny-like Mrs. Fieldmouse (the character who was later retconned as Felicity Fieldmouse). The debut consisted of the boys getting into all sorts of trouble around Mickey's home, until Mickey resorted to restraining and reading stories to the boys to keep them calm.
The twins' first film appearance was the 1934 Mickey Mouse cartoon Mickey's Steamroller. Aside from a cameo in 1938's Boat Builders, they didn't appear in animation again until Mickey's Christmas Carol (1983), with Morty playing the part of Tiny Tim and Ferdie as the not-named-onscreen Peter Cratchit.
In pre-World War II Mickey children's storybooks produced by the Disney studio, the nephews were usually called Morty and Monty rather than Morty and Ferdie. Very early books contain three or more nephews with various names, including Maisie and Marmaduke. Morty's name, short for Mortimer, is possibly in reference to the original planned name for Mickey, Mortimer Mouse, and Ferdie (sometimes misspelled "Ferdy") is short for "Ferdinand."
Ferdie disappeared from the Mickey Mouse comic strip in 1943, because Gottfredson thought the nephews were too much alike. He had plans to bring Ferdie back later as a bespectacled, intellectual, bookworm mouse with an Eton hat and coat with the explanation that he had been away at school. However, Gottfredson never got around to bringing Ferdie back and Morty remained in the strip alone. Morty was occasionally depicted with his best friend named Alvin and a sweetheart named Millie. Both were anthropomorphic dogs. Ferdie never vanished from comic book stories, however.
In recent years, some of Morty and Ferdie's comic book appearances have portrayed them as football players on the team Riverside Rovers. Their mother is depicted as a supportive "Soccer Mom." Morty & Ferdie are also occasionally pitted against their antagonists Pete's twin hellion nephews, Pierino & Pieretto.
Morty and Ferdie appeared in the extended Halloween episode, "The Scariest Story Ever: A Mickey Mouse Halloween Spooktacular". In the special, they go trick-or-treating with Huey, Dewey, and Louie and listen to Mickey's scary stories.
- Contrary to what was written in their profile in the book Disney Dossiers, they do not appear in Mickey's House of Villains, though it is possible they could be among the mouse orphans (with whom they share the same character design) seen in Gulliver Mickey, Orphans' Benefit, and Orphans' Picnic.