In the film, Mrs. Hasagawa is pursued by Nani for a job at her fruit stand. However, Mrs. Hasagawa does not understand Nani and it takes a few times for Nani to remind Mrs. Hasagawa of her ad in the paper. Nearby, a hula practice between Lilo with Stitch sends the alien twirling into Mrs. Hasagawa. They collide into the fruit stands and Mrs. Hasagawa gets a watermelon stuck on her head with Stitch poking out on top.
Mrs. Hasagawa makes a few appearances in the series. In "Mrs. Hasagawa's Cats", it is revealed that she keeps many experiments, thinking they are cats at her house. Most of these experiments are activated when Mrs. Hasagawa mistakes their pods for apricots and washes them to eat.
In the film, it is revealed that Mrs. Hasagawa has yet another experiment under her care, a cloud-like experiment that helps her at work by watering her fruit at the stand.
Mrs. Hasagawa owns fifteen experiments whom she took in, thinking they were cats and one that works with her at her fruit stand. Most of these experiments were activated when Mrs. Hasagawa mistook their pods for apricots and washed them to eat. They include:
- In the intro sequence of the series, Mrs. Hasagawa appears in two squares in the hula dance, with her image and movements mirrored to one another.
- Stitch mispronounces her name as "Hasagasawa".
- Mrs. Hasagawa has experiments from every series with the exception of the 4-series and 6-series.
- Aside from Hocker, the experiments under her care are not life-threatening, and most are only designed to frustrate their targets with mild inconveniences.
- It is unknown if she, being the caretaker of several alien life forms, is under the official protection of the United Galactic Federation as Lilo and Nani are.