Mysterio appears in the Ultimate Spider-Man episode "The Moon Knight Before Christmas" voiced by Paul Scheer. Quentin Beck is portrayed as an old enemy of Spider-Man and former stage magician who is presumed dead after an encounter with Spider-Man when he fell off the Brooklyn Bridge. Spider-Man dove into the river and only found Mysterio's helmet at the bottom of the river while his body is nowhere to be found. Since that incident, Spider-Man gave his helmet to Doctor Strange for safekeeping at the Sanctorum. Following his death, his daughter Frances Beck (voiced by Mary Kate Wiles) uses his helmet in an attempt to exact revenge on Spider-Man as a female Mysterio only to discover that Quentin Beck's soul is trapped within the helmet. Recalling Doctor Strange's saying "The Enemy of the Enemy" is my friend, Spider-Man casts the spell that would enable Frances' pursuer Moon Knight to enter when it comes to Mysterio summoning evil elves, giant gingerbread men, snow monsters created from snowmen, and animated toys. Using a specific wand that the Moon told him to wield, he stabs the helmet with the wand. Spider-Man pretends to follow the Moon by telling Moon Knight to free Frances from the helmet's control. After ending up inside the helmet where he sees Quentin in the helmet as a result of a deal with Dormammu. As Quentin states that his soul is lost, Spider-Man persuades Quentin not to give up. As a result, Quentin Beck, Frances Beck, and Spider-Man return to reality. Quentin and Frances later attend a dinner with Aunt May alongside Spider-Man and Moon Knight.