The crew is first seen on their ship, the Jolly Roger, in Neverland, singing "A Pirate's Life (Is a Wonderful Life)". Although they served under Captain Hook, it is implied that they grew bored of him, because they spent their time in the aforementioned song throwing swords at a crudely drawn picture labeled "Captain" that was drawn on the cabin door. When Smee, the first mate, comes out of the cabin, he wishes them a good morning, but they grab him by the shirt and tell him that there's nothing good about the morning because they're stuck on Neverland. Wishing to give up the quest for Peter Pan, they bully Smee into trying to convince Captain Hook to leave Neverland. One of them is then shown in the rigging with an accordion, singing (rather badly) until an annoyed Captain Hook offhandedly shoots him with a flintlock pistol for interrupting his concentration.
Later on, when Peter Pan, Wendy, John, and Michael return to Neverland, one of the crew spots them and Hook orders the crew to shoot the cannons at them. The crew tries to shoot at them, knowing that the sooner Peter dies, the sooner they all get to leave Neverland. Luckily, they manage to escape. Later on, after Captain Hook has an encounter with Peter Pan, he manages to capture his fairy friend, Tinker Bell and tricks her into telling him where his hideout, Hangman's Tree, is and then he locks her up in a lantern and takes the crew with him to Hangman's Tree with a bomb wrapped as a present for Peter from Wendy. When Hook, Smee, and the crew arrive there, they hear Wendy singing "Your Mother and Mine". Here, the crew, among with Smee, is shown to be visibly sad, implying that they lost their mothers at an early age and it was this that drove them to become pirates. When Wendy, John, Michael, and the Lost Boys come out to return home, they are captured by the crew, who take them back to the ship while Hook and Smee stay behind to put the bomb inside for Peter.
On the ship, the crew sings to the children about joining them or walking the plank. Wendy tells Captain Hook that they will never join them. They tie the boys up, take Wendy to the plank, and make her walk to the end and fell down to the sea. Captain Hook listen for the splash, but no sound comes. Unbeknownst to the crew, Peter saved Wendy just in time before she landed in the water, for he survived the bomb thanks to Tinker Bell going to his rescue upon escaping from the lantern upon learning of it. Still mad at his crew who were fearfully believed that the ship was bewitched, Captain Hook then throws one of them, Starkey, into the sea. He then learns that Peter is still alive. Then he and Peter start the sword fight. Peter released the boys to join the battle. Captain Hook order his crew to get them. John told the Lost Boys to fire the crew with their weapons, but the crew is ready to climb to the net to battle them. Tinker Bell gets Peter to tell him they are in trouble. Using his dagger, he flies to the net to cut the rope and the crew falls down to the rowboat with Smee and head to the sea. They're then rowing the boat away from Neverland while Captain Hook was swimming away by his nemesis, the crocodile.
In Return to Neverland, the crew still listens to Captain Hook and actually come with him to London to kidnap Wendy. However, they accidentally kidnap her daughter, Jane, (perhaps because they were not aware of the change of time since time has virtually stopped in Neverland). They follow Captain Hook's orders to the letter, as they join him in searching for Peter Pan and the Lost Boys. They also join him in the search of his treasure chest and the fight against Jane and the Lost Boys. Near the end of the film, the crew (along with Captain Hook and Smee) is chased away by the octopus (who imagines them all to be different kinds of fish).
- Main article: Sharky and Bones
Several members of Captian Hook's pirate crew appear in this film as secondary characters. Given names and speaking parts, this movie is functionally a prequal for the pirates prior to the events of Peter Pan. Several members of the crew are recognizable, despite the switch to 3D animation, post-Retun to Neverland. The only notable difference is the number of crewmates and their noticible youth.
Aside from Tinker Bell herself, this is the first occurance of Disney characters making the jump from 2-to-3-D animation. Following this, Ralph Breaks the Internet also incorperated previously 2-D characters.
The crew appears in the second season on the show, in flashbacks concerning Captain Hook. One is when he meets Milah, Rumplestiltskin's ex-wife loses his hand by facing the crocodile and the other is when he meets Baelfire at Neverland.
In Kingdom Hearts: Birth by Sleep, Smee is on the boat in Ventus' story of the game and he calls out to the crew to fire cannonballs at Ventus and Peter. The part of the crew in Kingdom Hearts is played by numerous Heartless instead of the original characters, presumably having been disposed of once Hook joined Maleficent's alliance.
The crew's Wasteland counterparts once lived in Tortooga until Captain Hook was forcibly converted into a Beetleworx at Skull Island and many of them were captured and converted as well. Smee and a few others, including Damien Salt and Scurvy Pat, escaped to Venture Land and have been trying to settle in there.
The crew is seen during the Peter Pan's Flight mini-game. They are first seen holding Cubby hostage in an attempt to learn the whereabouts of Peter Pan. When he and the player arrive, however, Captain Hook orders his crew to attack. They do so by blasting cannons at both Peter and the player. By reflecting the cannonballs, the player is able to defeat the crew and go after Captain Hook.
- Although the crew is implied to dislike Captain Hook's obsession to kill Peter Pan and their wish to leave Neverland to sail for treasure again in the original film, the sequel doesn't depict the crew complaining about Captain Hook's attempts to kill Peter and even enjoy aiding him on his plot to kidnap Peter and the Lost Boys despite still fearing Captain Hook, like most Disney henchmen fearing their boss (except for the villagers and Gaston's four stooges, who show no fear to Gaston in the original Beauty and the Beast, but not the live action remake). Thus, one can assume that after finding the treasure, the crew may have started to appreciate Captain Hook more than before and that maybe they grew to hate Peter over the years given his constant attacks on them.
- Another possibility is that they may be loyal to Captain Hook because he took them under his wing to teach them how to be pirates and that maybe Hook promised them to return to loot ships once they got rid of Peter Pan. In the deleted song "I'll Give You One Guess" from the second film, Jukes mentions that he was just a kid when Hook took him in and showed him a sailor's life, and Turk and Starkey then follow saying that Peter evaded them for so long and that now that they will get rid of him they will sail out to rob and plunder. As the song was deleted, however, the canon status of these statements is up for debate.
- Starkey, the crew member Captain Hook threw overboard, was his first mate and butler in the original novel, while Smee was the boatswain. Interestingly, in the original novel, he and Smee were the only two pirates to survive.
- The only pirate member names that were confirmed were Wibbles, Bill Jukes, Black Murphy, Mullins, Turk, Starkey, Skylights, and Damien Salt. It is unknown if all of the unnamed pirate crew members will have confirmed names from the play and/or novel.
- Mullins and 4 unnamed pirates are the only pirates who have patches on their right eye.
- It is unknown if Mullins's right eye was either damaged or not.
- The only pirate that died was Skylights, who played the accordion on top of Captain Hook's ship (much to the latter's annoyance that he shoots Skylights down).
- Jukes, the peglegged pirate in the Disney version, was described as a pirate with tattoos in the original novel. Also during the final battle in the play and novel, when he was given orders by Hook to fetch the cat o nine tails for the children, Jukes was either the first or second pirate to be killed by Peter.
- It is revealed that there are various members part of Hook's crew as shown in scenes along with the others.
- While the pirates tried to pursue John, Michael, and the lost boys, an unnamed one eye pink shirt pirate can be scene climbing along with Wibbles, Jukes, Turk, and the rest of the pirates (offscreen) and would later fall, along with all of the other pirates of the crew after Peter cuts the rope.
- The rest of the crew does not appear in "Jake and the Neverland Pirates", which presumably took place after Peter Pan.
- It could be implied that one eye pink shirt pirate is his twin brother since most of them look alike, both of them have a patch on their right eye.
- Many of the pirates are likely descended from other foreign countries as presumed by some of their accents and mannerisms: Turk appears to be Indian, Arabian, or even Moraccan, Black Murphy seems to be French or Canadian, Skylights seems to be Italian, Wibbles seems to be Swedish, Mullins appears to be German, and Jukes seems to be Scottish or Irish.
- As shown in "Your Mother and Mine", it could be implied that all the pirates in the crew (as well as Mr. Smee) lost their mothers at a young age and/or missed them so dearly.