- “To play a simple tune, you will have to use four horns of different lengths.”
- ―Professor Owl
Utilizing archive footage from the original shorts (as well as new animation), Professor Owl would become the host of the early videos in the Sing Along Songs series, leading his classroom in the theme song of the video series in addition to his various transition segments. As the series went on, Professor Owl was phased out as the host of the videos, instead only appearing at the beginning to introduce either Jiminy Cricket or Ludwig Von Drake as the video's host. In the All-Time Favorites videos and the beginning of the "Honor to Us All" video, the Professor was only heard in voice over.
- The character Merlin in Legend of the Three Caballeros bares a striking resemblance to Professor Owl.
- The shape of Professor Owl, among other things, served as the inspiration for the character of KnowsMore in Ralph Breaks the Internet.