- “Oh, would you just shut up?! You're rats with wings!”
- ―The seagulls' only word
In the film, they are portrayed as greedy, mindless eating machines who only shout one word: "Mine!"
They are first seen cawing "Mine!" while Nigel has a crab in his wing that he is going to eat, much to his irritation. However, he ends up relieving the crab when he overhears from his fellow pelicans that Nemo's father, Marlin, is trying to search for his son, although the crab manages to escape from the Seagulls.
Later, the Seagulls surround Nigel, Marlin and Dory, and also nearly try to eat Marlin and Dory while trying to escape from Nigel (as they thought he was going to try and eat them when all he was trying to do was tell them that he knows Nemo and where he is), but Nigel saves them. They chase after Nigel until their beaks crash into a sail of one of the sailboats.
The Seagulls make their last appearance when Dory and Nemo try to get information out of a crab about where he saw Marlin go (Dory intended to feed the crab to the Seagulls if he didn't answer them).
A similar flock of seagulls briefly appears during the climax of the film, still saying "Mine! Mine! Mine!", when Dory and Hank steal the Cleveland Aquarium truck by scaring off the truck drivers.
Three of the Seagulls appear on top of a buoy as animatronics in the Finding Nemo Submarine Voyage lagoon. They periodically flap their wings and say "Mine! Mine! Mine!"
Three of the Seagulls reside on the rocks outside the entrance to The Seas with Nemo & Friends at Epcot. Like their Disneyland counterparts, they periodically flap their wings and say "Mine! Mine! Mine!"
At Finding Nemo: The Musical in Disney's Animal Kingdom, they do not actually appear in the show itself, but they do appear in the bubbles flanking the stage after the show ends to say "Bye! Bye! Bye!"
- The Seagulls bear some resemblance to Feathers McGraw, the antagonist penguin from the Wallace and Gromit movie "The Wrong Trousers".
- A different flock of seagulls resembling the ones from the first film make an appearance near the end of Finding Dory when Dory and Hank drive the truck containing the quarantined fish into the ocean. Like the other flock, all they say is "Mine! Mine! Mine!"
- In the Polish dub of the movie, the Seagulls say, "Daj! Daj! Daj!" (pronounced like the English word "die"), which actually means "Give" in English.
- Some seagulls also make a cameo appearance in LEGO The Incredibles throughout the level "Above Parr". In this game, they are shown as regular LEGO seagulls, but still say, "Mine! Mine! Mine!"
- The Seagulls are rivals with the pelicans because they tried to protect the other fishes from being eaten.
Disney Parks and other live appearances