The Seeonee pack is led by Akela, the pack alpha. The commander of a large pack, Akela ensures that all his charges obey the law of the jungle. Amongst his many pack-mates are his mate Leah, Raksha and her son Grey Brother, and Rama, Raksha's mate. Bagheera the panther serves as an honorary council member for the wolf pack due to his strong friendship with the pack.
The Seeonee pack's role in the 1967 film is minor; most of the wolves are not seen until Akela summons them to discuss the return of the man-eating Shere Khan to their part of the jungle. The members seen most often until this point are Raksha, Rama and their pups, who adopt and look after Mowgli after Bagheera leaves him in their care, knowing they can protect him from harm. When word reaches the pack of Shere Khan returning to their part of the jungle, Akela calls a meeting of all his wolves and all agree that Mowgli must leave for his own safety. Though Rama believes Mowgli is entitled to the pack's protection, Akela reminds him that even the strength of the pack cannot match Shere Khan's. Bagheera then settles the matter by saying that he will take Mowgli to the Man-village that he knows of, settling the dispute. Akela wishes Bagheera luck and the pack watches as he leaves to take Mowgli to safety. They are not seen again for the rest of the film.
In Jungle Cubs, Akela and his new mate, Leah, form the Seeonee pack when they leave Cain's pack in the episode "The Coming of the Wolves". During the events of this episode, Leah drew the attention of Cain, but spurned his romantic advances, causing them to run away together. Though they end up in a falling-out with the cubs upon meeting them (due to the wolves taking their lair), they soon band together to face Cain and his pack, with Akela eventually defeating Cain in battle. After, Leah gives birth to eight pups and the pair name Bagheera, Shere Khan, Hathi, Kaa and Louie alike as the pups' godfathers.
The wolves in this live action remake were mostly portrayed by wolfdogs. Scenes featuring these characters were filmed at Fall Creek Falls State Park in Spencer, Tennessee.
After Mowgli and Grey Brother find themselves lost in the jungle, the panther Bagheera leads the two across the lush environment where in the waterfall is a wolf pack frolicking across the scenery followed the pack happily staring at Mowgli knowing that they saw the man-cub again. Over lifetime, Mowgli continues adapting his life in the jungle with the Seeonee wolves following him as he continues transitioning from boy to man.
When a grown-up Mowgli returns to the jungle, the Seeonee Pack then runs along with Mowgli, Grey Brother, and Bagheera in a plan to find Dr. Julius Plumford to save Baloo's life only to find out the doctor left with Kitty and her father Colonel Brydon. Mowgli then takes William Boone and his group to explore the jungle around them, just as the Seeonee Pack and Bagheera spy on Mowgli bringing humans to the jungle, believing that Boone and his soldiers are a threat to the jungle. After escaping King Louie's temple and Shere Khan seeing that Mowgli is not harming the jungle animals, Mowgli takes Kitty to the Seeonee Pack where the wolves stare at Mowgli and Kitty falling in love just as Mowgli becomes the protector of the jungle at the end of the film.
The Seeonee wolf pack's role in the film is much greater; they are actively shown training Mowgli to learn the ways of the wolves and the laws of the jungle alongside their younger members, with Bagheera serving as their mentor. The wolf pack is again led by Akela, with Raksha serving as Mowgli's mother and protector and Grey Brother, still a puppy, serving as Mowgli's adoptive sibling. During the drought in an event known as the Water Truce, the Seeonee pack comes to drink at the watering hole peacefully alongside the other animals. Mowgli's inventive tricks during their visit unnerve the pack, causing Akela to gently reprimand the boy.
When Shere Khan comes to the watering hole, tension arises when the tiger states that man is forbidden in the jungle, including the man-cub. Though he respects the sanctity of the Water Truce, he asks the pack how many lives Mowgli is worth and to give him an answer by the start of the rainy season before leaving. This leaves the pack divided on whether Mowgli should stay or go, causing Mowgli to leave so he can ensure their safety. Though Raksha protests to this, she agrees in the end that it is the only way to protect him.
When next the pack is seen, Shere Khan comes demanding the Man-cub, only for Akela to say that he has left to be with other humans. Deciding to lure the boy back out, he kills Akela by throwing him off a cliff and instates himself as their new leader, telling the wolves to spread the word of Akela's death so that it reaches Mowgli's ears. During his time as leader, Shere Khan also punishes Raksha by subtly threatening her pups and trying to influence them into hating humans through use of stories.
When Mowgli brings fire into the forest, the Seeonee pack and the other animals gather at the watering hole and see the boy face Shere Khan. Inspired by his refusal to fight Shere Khan with fire after seeing that he accidentally caused a forest fire, the wolves, alongside Baloo the bear and Bagheera, fight Shere Khan to give Mowgli enough time to set a trap in the burning jungle and kill Shere Khan, ending the tiger's reign of terror. They then salute him with howls for his victory over Shere Khan and his calling the elephants to help put out the fire. With peace restored, Raksha takes Akela's place as alpha wolf and re-accepts Mowgli into the wolf pack.
- The Seeonee pack's role in the books is great; among the many feats they accomplish are their raising of Mowgli, helping him to get revenge on Buldeo for branding him a witch, and defeating the Dholes, red dogs that destroy everything in their paths.
- Shere Khan has a much more prominent influence on the young wolves of the Seeonee pack in the books.
- The name of "Seeonee" is an intentional misspelling by Rudyard Kipling, and the name comes from the area of India called the Seoni region, which is located in Madhya Pradesh.