The name of the club reminds the main instruments of comic and cartoon creation and suggests the nature of its workers.
According to Mr. Maroon, the club is managed by Toons, but clients are exclusively human. Penguin waiters attend at the tables, keeping a small degree of toon humor: for example, when Eddie orders a drink "on the rocks", they put a real piece of rock in the glass instead of ice. The club allows clients to smoke when attending the shows, as suggested by Betty Boop selling cigars and cigarettes.
While drinking at the tables, customers can enjoy some sketches performed by toons, both comical and purely musical: Donald and Daffy Duck regularly perform a piano play which, thanks to their tempers and according to regular customer Marvin Acme, they never managed to finish: however, seems like watchers enjoy it more for their fights than rather their piano play.
Undeniably, the main event of the show is Jessica Rabbit's performance of her song, something always very appreciated by the audience. Betty Boop claims Acme never misses it.
In Who Framed Roger Rabbit, Eddie Valiant is sent to the Ink and Paint Club by R.K. Maroon in order to investigate some rumors about Roger Rabbit's wife which are causing the cartoon star to lower his performance. Here, Eddie has his first and only meeting with jovial prank tycoon Marvin Acme, Toontown's owner. Together, the two assist at Jessica's singing. After the song, Jessica and Marvin retire in her room and Eddie tries to spy them, but he's stopped by Bongo and thrown out; however, he manages to find a window and photograph them for Roger.
The Club is not seen again, but Roger mentions to have returned there to confront his wife and he found a piece of paper which he used to write a love letter to Jessica. That paper later was revealed to be Marvin Acme's will.