After a night of trick or treating, Mickey, Donald and Goofy take Mickey's and Donald's nephews back to the mouse's decorated home, where they count all the candy they gathered over the night. At "the witching hour", Mickey decides to tell the kids a scary story and everyone sits on the couch while he sets the mood: At the castle of "Dr. Goofenstein", "Duckgor" finds the body parts needed for Dr. Goofenstein's experiment. They bring to life a giant Frankenstein Monster (comically depicted with Mickey's head) who promptly comes to life and begins happily singing and dancing to a Broadway/disco number.
The kids are obviously unimpressed with Mickey's feeble attempt at a scary story as it wasn't horror scary but comedy horror and he apologizes promising to tell a "real" scary story. However, Goofy pushes him aside and claims that in order to tell a scary story he needs "visual aids" and tries to tell one about a swamp monster by using a cheap sock puppet. Scared by his own puppet, Goofy jumps and hides in the couch, forcing Donald to take over by using a flashlight. His attempt fails as well, as the kids cannot understand his quack-moaning, so Mickey decides to try again.
Long ago, the world is in danger of the Prince of Vampires. The only people who can stop him are "Van Mousing" and his partners "Dipworth Goofington III" and "Drake McQuack". The three, armed with their weapons of choice (Mousing with his crossbow, McQuack with his baseball bat and Goofington with his garlic and "steaks"), make it through the skeleton labyrinth and past two werewolves before they come face to face with the Prince of Vampires. The trio heroically battle him, with Van Mousing using the coming sunlight to defeat him. However, during the battle, Van Mousing is bitten, turning him into a vampire (thankly off-screen).
The kids are still unimpressed with Mickey's story as they all saw the ending coming and he, Donald and Goofy retreat into the kitchen. Goofy and Donald tell Mickey that he is incapable of telling a scary story due to him being a happy-go-lucky kind of guy and he solemnly agrees with them. As he goes out to tell the kids that he is finished, he sees that they have become rowdy and wild. Donald and Goofy lock Mickey in the room to face the disobedient children who insult him and his lederhosen costume. Mickey, having to finally snapped by this, sets the kids down to tell one more scary story, with Donald and Goofy coming in to listen as well.
Five children (represented as Huey, Dewey, Louie, Morty and Ferdie wearing lederhosen, much to their dismay) are constantly stealing pies in their village which is famous for pies and the villagers are fed up with the kids' pie steeling. One day, they are attracted by the scent of an unusual pie owned by an old lady. They steal it from her and eat it, agreeing that it is the best pie they have ever tasted. They find the old lady's house and split up to look for the pie. Within the house, all the kids are all captured while Huey finds the pie. He is discovered by the old lady who reveals that her secret ingredient are "the most snot-nosed, rotten little boys" she could find and transforms into a green skinned witch, who chases and grabs Huey to use in her pie, caputing all five of the rotten children and making them into the desert food they loved most of all, ready to eaten themselves and never be seen or heard from again.
The story finally and successfully scares the kids, as well as Donald and Goofy, who all run upstairs and hide under the blankets in bed. Mickey collects the candy, having to finally succeed in scaring the kids, just as the door knocks. Mickey goes to the door to pass out to a trick or treater, only to see that it is the witch from his story with a pie. Mickey screams and runs upstairs to join the rest. Now official frighted, the boys admit the story Mickey told was great and decided to not hear anymore scary stories before hiding under the covers. The witch turns out to be Minnie, who is just wearing a costume of a witch and has arrived with Daisy to greet the boys. Both are confused by Mickey's response, with Daisy asking what kind of pie she had made. When Minnie reveals that it's made from pumpkin, a bunch of jack-o'-lanterns Mickey had placed in his house scream in terror be the reponse and passed out with their lantern smoke spelling out "The End".
- Mickey Mouse
- Donald Duck
- Morty and Ferdie Fieldmouse
- Huey, Dewey, and Louie
- Minnie Mouse
- Daisy Duck
- Mrs. Johnson
- Horace Horsecollar (cameo)
- Clarabelle Cow (cameo)
- Clara Cluck (cameo)
- Chernabog (cameo)
- Dirty Bill (cameo)
- Headless Horseman (cameo as statue)
- Chris Diamantopoulos - Mickey Mouse, Morty Fieldmouse, Ferdie Fieldmouse, The Prince of Vampires
- Tony Anselmo - Donald Duck
- Bill Farmer - Goofy
- Russi Taylor - Huey, Dewey, and Louie, Minnie Mouse, Granny Hexobah
- Tress MacNeille - Mrs. Johnson, Daisy Duck
- This is the second thirty-minute special of the series, following Duck the Halls: A Mickey Mouse Christmas Special.
- The special marks the first appearance of Mickey's nephews, Morty and Ferdie, in the series, and their first appearance in animation since their roles as Tiny Tim and Peter Cratchit in Mickey's Christmas Carol.
- This is the third time Goofy's alter ego, Super Goof, has appeared in television, albeit as a costume instead of Goofy assuming the actual role.
- Mickey’s lederhosen costume is the same one he wears in the episode "Yodelberg".
- The town in which the special takes place is modeled after the town in Trick or Treat.
- Huey, Dewey, and Louie's costumes are the same costumes they wear in the short.
- Furniture from the Haunted Mansion appears in Mickey's house as Halloween decorations, such as the Demon Clock and the Pipe Organ.
- The Steamboat from Steamboat Willie briefly appears outside Mickey's window.
- A portrait of the Twins from Duck the Halls: A Mickey Mouse Christmas Special can be seen on the wall of Mickey's house.
- The six children who are trick or treating in the beginning at the special were dressed up as the Lost Boys from Peter Pan.
- Chernabog from the "Night on Bald Mountain" segment of Fantasia makes a cameo at the beginning of the special.
- Before the "Goofenstein", Mickey steps in front of a curtain and warns the kids that the story is going to be scary. This is a reference to the beginning of the 1931 film Frankenstein, where Edward Van Sloan warned the audience about the film they were going to see. Mickey quotes his lines almost word-for-word.
- This episode is the final time Russi Taylor was able to voice Huey, Dewey, and Louie before passing away in the Summer of 2019.